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商品详细Dojindo/-硫生物-SSP4/SSP4/SB10
Dojindo/-硫生物-SSP4/SSP4/SB10
Dojindo/-硫生物-SSP4/SSP4/SB10
商品编号: SB10
品牌: 同仁
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产地: 美国(厂家直采)
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产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
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商品介绍
DescriptionReferencesDataManualS.D.S

Product Description:It becomes obvious that there are a lots of molecules containing sulfane sulfurs such as persulfides and polysulfides in living organism. These molecular species are involved in production, storage and release of hydrogen sulfide, which is recognized as an important physiological mediator. Furthermore, recent studies reveal that persulfides and polysulfides may control intracellular signal transduction through s-sulfhydration of proteins.SSP4 (Sulfane Sulfur Probe 4) is a novel fluorescent probe to detect sulfane sulfurs selectively. SSP4 itself is non-fluorescent, but it emits strong green fluorescence when it reacts with sulfane sulfurs. Thus, SSP4 enables high sensitive fluorescence detection and imaging of sulfane sulfurs.

Fig. 1 Chemical Reaction with sulfane sulfer

Fig. 2 Fluorescence spectrum (Em: 482nm/Ex: 515nm)


Chemical species containing sulfane sulfurs:

Fig. 3 The formation of sulfane sulfer in vivo changes due to reduction/oxidation and translation

1. W. Chen, C. Liu, B. Peng, Y. Zhao, A. Pacheco, and M. Xian,“New fluorescent probe for sulfane sulfurs and the application in bioimaging“, Chem. Sci., 2013, 4, 2892.2. T. Ida, T. Sawa, H. Ihara. Y. Tsuchiya, Y. Watanabe, Y. Kumagai, M. Suematsu, H. Motohashi, S. Fujii, T, Matsunaga, M. Yamamoto, K. Ono, N. O. Devarie-Baez, M. Xian, J. M Fukuto, and T. Akaike, “Reactive cysteine persulfides and S-polythiolation regulate oxidative stress and redox signaling”, Proc Natl Acad Sci U S A., 2014, 111, 7606.3. E. Marutani, M. Sakaguchi, W. Chen, K. Sasakura, J. Liu, M. Xian, K. Hanaoka, T. Nagano, and F. Ichinose, “Cytoprotective effects of hydrogen sulfide-releasing N-methyl-D-aspartate receptor antagonists mediated by intracellular sulfane sulfur”, Med. Chem. Commun., 2014, 5, 1577.4. M. Sakaguchi, E. Marutani, H-S. Shin, W. Chen, K. Hanaoka, M. Xian, and F. Ichinose, “Sodium Thiosulfate Attenuates Acute Lung Injury in Mice”, Anesthesiology. 2014,121, 1248.

Cell Imaging:

Left: Control cellsRight: Treatment of 100 μmol/l Na2S3

Cell: CHO cellExposure time: 1,000 msc

Related Categories Sulfur Biology Oxidative Stress Assay

品牌介绍
Dojindo细胞分析细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐,称为WST。WST-8是高度稳定的WST,用于Cell Counting Kit-8(CCK-8)。由于WST-8甲maz是水溶性的,因此不会形成晶体。因此,不需要诸如MTT测定的增溶过程。此外,CCK-8的检测灵敏度高于其他四唑盐,例如MTT,XTT,MTS或WST-1。WST检测机制 ß-半乳糖苷酶检测试剂细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢应用产品展示细胞生长检测,药物筛选,比色/荧光检测细胞计数试剂盒-8细胞计数试剂盒8 + 96孔有机硅定向剂细胞计数试剂盒-F细胞毒性LDH检测试剂盒-WST 96孔有机硅定向剂MTT了解检测机制的差异:点击这里细胞周期分析细胞周期测定溶液深红色细胞周期测定溶液蓝色