DescriptionReferencesS.D.S
Reaction Scheme
Product DescriptionNitro-TB is dissolved by hot water or hot methanol. Other organic solvents such as acetone and ether cannot solubilize Nitro-TB. Nitro-TB is easily reduced by dehydrogenase to purple formazan dye aggregates. It is used for dehydrogenase detection on agarose gels.
1. K. Tsou, et al., Synthesis of Some p-Nitrophenyl Substituted Tetrazolium Salts Electron Acceptors for the Demonstration of Dehydrogenases. J Am Chem Soc. 1956;78:6139-6144.2. J. R. Baker, et al., Mechanism of Fructosamine Assay: Evidence against Role of Superoxide as Intermediate in Nitroblue Tetrazolium Reduction. Clin Chem. 1993;39:2460-2465.
Related Categories Redox Dyes
