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商品详细Dojindo/TOOS/1/OC13
Dojindo/TOOS/1/OC13
Dojindo/TOOS/1/OC13
商品编号: OC13
品牌: 同仁
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
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商品介绍
DescriptionApplicationReferencesS.D.S
Oxidation Reaction

Preparation of Assay Solution – TOOS1. Dissolve 20 mg TOOS with 10 ml PBS to prepare 6.6 mM TOOS solution.2. Dissolve 14 mg 4-aminoantipyrin (AA) with 10 ml PBS to prepare 6.6 mM 4-AA solution.3. Prepare 2 U/ml horseradish peroxidase solution with PBS.4. Mix the same volume of each solution together to prepare assay solution. Store the assay solution at 4°C with protection from light.

Assay Protocol1. Prepare sample solutions for the enzymatic oxidation reaction. The pH range of the buffer solution should be from 5.5-9.5.2. Prepare standard solutions containing known amounts of substrate using the same buffer.3. Add the appropriate units of oxidase to the sample solution, followed by an addition of the same volume of the assay solution.4. Incubate the mixture at room temperature or at 37oC for 30 min to 1 hour.5. Measure the O.D. at 555 nm.6. Prepare a standard curve, and determine the substrate concentration in the sample solution.

1. K. Tamaoku, et al., New water-soluble Hydrogen Donors for the Enzymatic Photometric Determination of Hydrogen Peroxide. II. N-Ethyl-N-(2-hydroxy-3-sulfopropyl)aniline derivatives. Chem Pharm Bull. 1982;30:2492-2497.2. K. Tamaoku, et al., New water-soluble Hydrogen Donors for the Enzymatic Spectrophotometric Determination of Hydrogen Peroxide. Anal Chim Acta. 1982;136:121-127.3. B. C. Madsen, et al., Flow Injection and Photometric Determination of Hydrogen Peroxide in Rainwater with N-Ethyl-N-(sulfopropyl)aniline sodium salt. Anal Chem. 1984;56:2849-2850.

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品牌介绍
Dojindo细胞分析细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐,称为WST。WST-8是高度稳定的WST,用于Cell Counting Kit-8(CCK-8)。由于WST-8甲maz是水溶性的,因此不会形成晶体。因此,不需要诸如MTT测定的增溶过程。此外,CCK-8的检测灵敏度高于其他四唑盐,例如MTT,XTT,MTS或WST-1。WST检测机制 ß-半乳糖苷酶检测试剂细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢应用产品展示细胞生长检测,药物筛选,比色/荧光检测细胞计数试剂盒-8细胞计数试剂盒8 + 96孔有机硅定向剂细胞计数试剂盒-F细胞毒性LDH检测试剂盒-WST 96孔有机硅定向剂MTT了解检测机制的差异:点击这里细胞周期分析细胞周期测定溶液深红色细胞周期测定溶液蓝色