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商品详细Dojindo/DAB/5/D006
Dojindo/DAB/5/D006
Dojindo/DAB/5/D006
商品编号: D006
品牌: 同仁
市场价: ¥14840.00
美元价: 8904.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionApplicationReferencesS.D.S

Product DescriptionDAB is one of the most commonly used oxidative chromogenic dyes for the detection of peroxidase in immunohistochemistry. In the presence of H2O2 and peroxidase, DAB is oxidized into a brown pigment. These brown pigments are aniline black type compounds and are firmly deposited around peroxidase on cell membranes or tissues. Most DAB available on the market appears brown as a result of oxidation during processing or storage. On the other hand, Dojindo offers a high quality DAB with a white or slightly reddish-gray powder appearance that is good for sharp staining.

Preparation of Sample Staining Solution1. Dissolve 9 mg DAB with 1 ml PBS to prepare 100X DAB solution.2. Mix 5 μl of 30% hydrogen peroxidase solution with 1 ml PBS to prepare 200X H2O2 solution.3. Add 10 ul of 100X DAB solution and 5 ul of 200X H2O2 solution to 1 ml PBS to prepare staining solutiona).4. Add the staining solution to a sample in a staining chamber, and incubate the sample at room temperature for 5 minutes to 1 hourb).5. Wash the sample with PBS several times to stop the staining reaction.

a)The staining solution is not stable. Prepare fresh solution prior to use.b)For better staining, incubate the sample with 0.09 mg DAB/PBS solution for 10 minutes before adding the staining solution.

1. A. B. Novikoff, et al., Studies on Microperoxisomes V. Are Microperoxisomes Ubiquitous in Mammalian Cells. J Histochem Cytochem. 1973;21:737.2. V. Herzog, et al., A New Sensitive Colorimetric Assay for Peroxidaase Using 3, 3 EDiaminobenzidine as Hydrogen Donor. Anal Biochem. 1973;55:554.3. H. Yamada, et al., Improvement of Technique of Immunohistochemical Demonstraction of Bioactive Substances in the Central Nervous System. Acta Histochem Cytochem. 1987;20:629.4. K. L. Cheng, Determination of Traces of Selenium 3,3 EDiaminobenzidine as Selenium(IV) Organic Reagent. Anal Chem. 1956;28:1738.

Related Categories

Cell Staining Redox Dyes
品牌介绍
Dojindo细胞分析细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐,称为WST。WST-8是高度稳定的WST,用于Cell Counting Kit-8(CCK-8)。由于WST-8甲maz是水溶性的,因此不会形成晶体。因此,不需要诸如MTT测定的增溶过程。此外,CCK-8的检测灵敏度高于其他四唑盐,例如MTT,XTT,MTS或WST-1。WST检测机制 ß-半乳糖苷酶检测试剂细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢应用产品展示细胞生长检测,药物筛选,比色/荧光检测细胞计数试剂盒-8细胞计数试剂盒8 + 96孔有机硅定向剂细胞计数试剂盒-F细胞毒性LDH检测试剂盒-WST 96孔有机硅定向剂MTT了解检测机制的差异:点击这里细胞周期分析细胞周期测定溶液深红色细胞周期测定溶液蓝色