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当前位置: 首页 > 产品中心 > peptide > Dojindo/1-甲氧基PES/50/M470
商品详细Dojindo/1-甲氧基PES/50/M470
Dojindo/1-甲氧基PES/50/M470
Dojindo/1-甲氧基PES/50/M470
商品编号: M470
品牌: 同仁
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionDataS.D.S

Product Description

1-Methoxy PES is an electron mediator which has higher stability of solutions than 1-Methoxy PMS(M003). The stability in neutral to alkali conditions has been extremely improved with 1-Methoxy PES. 1-Methoxy PES is a stable small-molecular compound and it has an equal or higher thermal stability than diaphorase. The 1-Methoxy PES solution can be stored long term.

Characteristics of Each Electron Mediator

Stable in Wide Range of pH – Comparison with 1-Methox PMS

Methods:The residual activity of electron mediator was determined by colorimetric measurement after mixing WST-8 and NADH to the electron mediator solution that was stored for 30 days at 30 oC.

Result:The stability of 1-Methoxy PMS solution dropped drastically at pH6 and above, on the other hand, 1-Methoxy PES solution was stable until pH8.

Solution Stability Compared to Diaphorase

Methods:Working solution:1mmol/l WST-850 mmol/l HEPES buffer (pH7.0)1% Triton X-1001-Methoxy PES (final concentration of 2 umol/l) or diaphorase (final concentration of 0.05 U/ml) was mixed with the working solution. 100 ul of 200 umol/l NADH (50 mmol/l HEPES buffer pH7.0) was added to 100 ul of each electron mediator solution. After 20 minutes at 37 oC, an absorbance of WST formazan was read (450nm). The freshly prepared solution and the solution stored for 45 days at 5oC were utilized for residual activity comparison.

Result:There was 20% drop in the residual activity of diaphorase after 45 days of storage, on the other hand, 1-Methoxy PES showed similar residual activity as a freshly prepared solution.

Related Categories Cell Proliferation / Cytotoxicity Redox Dyes

品牌介绍
Dojindo细胞分析细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐,称为WST。WST-8是高度稳定的WST,用于Cell Counting Kit-8(CCK-8)。由于WST-8甲maz是水溶性的,因此不会形成晶体。因此,不需要诸如MTT测定的增溶过程。此外,CCK-8的检测灵敏度高于其他四唑盐,例如MTT,XTT,MTS或WST-1。WST检测机制 ß-半乳糖苷酶检测试剂细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢应用产品展示细胞生长检测,药物筛选,比色/荧光检测细胞计数试剂盒-8细胞计数试剂盒8 + 96孔有机硅定向剂细胞计数试剂盒-F细胞毒性LDH检测试剂盒-WST 96孔有机硅定向剂MTT了解检测机制的差异:点击这里细胞周期分析细胞周期测定溶液深红色细胞周期测定溶液蓝色