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商品详细多金多/DTNB/1/D029
多金多/DTNB/1/D029
多金多/DTNB/1/D029
商品编号: D029
品牌: 同仁
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionReferencesS.D.S

Product DescriptionDTNB is known as Ellman’s reagent. It is used for the colorimetric determination of thiol groups in biological samples. It is fairly soluble in water. Colorless DTNB is converted to yellow 5-Mercapto-2- nitrobenzoic acid in the presence of thiol compounds (Fig. 1). As 5-Mercapto-2-nitrobenzoic acid has an absorption maximum at 412 nm, the absorption spectrum of DTNB does not interfere with thiol detection (Fig. 2).

Fig. 1 DTNB reaction with thiol compound

Fig. 2 Absorption spectra of DTNB and reduced DTNB

1. D. R. Grassetti, et al., Determination of Sulfhydryl Groups with 2, 2 E or 4, 4 EDithiodipyridine. Arch Biochem Biophys. 1967;119:41-49.2. R. E. Humphrey, et al., Spectrophotometric Determination of Sulfite with 4, 4 EDithiodipyridine and 5, 5 EDithiobis-(2-nitrobenzoic acid). Anal Chem. 1970;42:698-702.3. R. E. Humphrey, et al., Spectrophotometric Determination of Cyanide with Organic Disulphides. Talanta. 1971;18:491-497.4. T. Uete, et al., Spectrophotometric Micromethod for Measuring Cholinesterase Activity in Serum or Plasma. Clin Chem. 1972;18:454-458.

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