Product Description2-PDS and 4-PDS are used for the photometric determination of thiol groups in biological samples. These compounds react with the thiol groups at pH 5 to form mercaptopyridine (Fig. 1 and 2). The maximum wavelengths of 2-Mercaptopyridine and 4-Mercaptopyridine are 343 nm and 324 nm, respectively.
Fig 1. 4-PDS reaction with thiol compound
1. D. R. Grassetti, et al., Determination of Sulfhydryl Groups with 2, 2 E or 4, 4 EDithiodipyridine. Arch Biochem Biophys. 1967;119:41-49.2. R. E. Humphrey, et al., Spectrophotometric Determination of Sulfite with 4, 4 EDithiodipyridine and 5, 5 EDithiobis-(2-nitrobenzoic acid). Anal Chem. 1970;42:698-702.3. R. E. Humphrey, et al., Spectrophotometric Determination of Cyanide with Organic Disulphides. Talanta. 1971;18:491-497.4. T. Uete, et al., Spectrophotometric Micromethod for Measuring Cholinesterase Activity in Serum or Plasma. Clin Chem. 1972;18:454-458.
Related Categories SH Detection
