4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:主营:化学试剂,CCK-8试剂盒,细胞检测,自噬研究,化学标记,生化试剂等
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > peptide > Dojindo/2,3-二氨基萘(不检测)/10/D418
商品详细Dojindo/2,3-二氨基萘(不检测)/10/D418
Dojindo/2,3-二氨基萘(不检测)/10/D418
Dojindo/2,3-二氨基萘(不检测)/10/D418
商品编号: D418
品牌: 同仁
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionApplicationReferencesS.D.S

Product DescriptionThe Griess assay is a simple and popular method for detecting NO concentration. 2,3-Diaminonaphthalene (DAN) is a highly sensitive alternative to the Griess assay. The DAN method is 50-100 times more sensitive than the Griess assay: While the detection limit of the Griess assay is 1 mM, the limit of the DAN method is 10-50 nM. DAN reacts with NO2– in acidic conditions to produce fluorescent naphthalenetriazole. The wavelength of the emission maximum of naphthalenetriazole is 410 nm. However, detection at 450 nm is recommended to avoid fluorescent blanks and increase sensitivity. The fluorescent background of DAN is low for maximum sensitivity. The optimal reaction conditions of DAN with NO2– have been determined. The reaction should proceed at pH 2 at room temperature for 5 minutes, and the resulting fluorescence of naphthalenetriazole should be determined at a pH of 10 or more. DAN is a photosensitive reagent and sometimes becomes dark brown colored crystals. Since this brown product cannot be utilized for the fluorescent detection, recrystallization is necessary.

Reaction of 2,3-Diaminonaphthalene with NO2

NO2 Assay Using 2,3-Diaminonaphthalene (DAN)1. Dissolve 50 μg DAN in 1 ml 0.62 M HCl to prepare 0.31 mM DAN solution.a)2. Mix 10 μl DAN solution with 100 μl NaNO2 solution (0-10 mM) or sample solution. Incubate the mixture at room temperature for 10-15 minutes.3. Add 5 μl 2.8 M NaOH solution to the reaction solution.b)4. Dilute 100 μl of this solution with 4 ml water, followed by fluorescent measurement with excitation wavelength at 365 nm and emission wavelength at 450 nm.5. Prepare a calibration curve using this data where the X-axis is NaNO2 concentration and the Y-axis is fluorescence intensity. Then, use this calibration curve to determine the NO2 concentration of the sample solution.

a) Acidic conditions are required for a rapid reaction.b) Basic conditions (pH 10 or higher) are required for a high fluorescence signal.

C. R. Sawicki, Anal. Lett., 4, 761 (1971);P. Damiani, et al., Talanta, 8, 649 (1986);W. R. Tracey, et al., J. Pharmacol. Exp. Ther., 252, 922 (1990);J. S. Pollock, et al., Proc. Natl. Acad. Sci. USA, 88, 10480 (1991);T. P. Misko, et al., Anal. Biochem., 214, 11 (1993);R. G. Tilton, et al., Invest. Ophthamol. Vis. Sci., 35, 3278 (1994);T. A. Mayer, et al., J. Surg. Res., 58, 570 (1995);Y. Kono, et al., Biochem. J., 312, 947 (1995);R. Metheringham, et al., Microbiology, 143, 2647 (1997);A. M. Rao, et al., Brain Res., 793, 265 (1998);N. Nakatsubo, et al., Biol. Pharm. Bull., 21, 1247 (1998);D. Jourd’heuil, et al., Arch. Biochem. Biophys., 365, 92 (1999).

Related Categories Oxidative Stress Array

品牌介绍
Dojindo细胞分析细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐,称为WST。WST-8是高度稳定的WST,用于Cell Counting Kit-8(CCK-8)。由于WST-8甲maz是水溶性的,因此不会形成晶体。因此,不需要诸如MTT测定的增溶过程。此外,CCK-8的检测灵敏度高于其他四唑盐,例如MTT,XTT,MTS或WST-1。WST检测机制 ß-半乳糖苷酶检测试剂细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢应用产品展示细胞生长检测,药物筛选,比色/荧光检测细胞计数试剂盒-8细胞计数试剂盒8 + 96孔有机硅定向剂细胞计数试剂盒-F细胞毒性LDH检测试剂盒-WST 96孔有机硅定向剂MTT了解检测机制的差异:点击这里细胞周期分析细胞周期测定溶液深红色细胞周期测定溶液蓝色