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商品详细Dojindo/间谍LHP/1/S343
Dojindo/间谍LHP/1/S343
Dojindo/间谍LHP/1/S343
商品编号: S343
品牌: 同仁
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionReferencesS.D.S
Product DescriptionSpy-LHP is a newly developed fluorescent probe for live cell imaging of phospholipid peroxide. There are several detection methods available for lipid peroxides, such as iodide titration method, colorimetric method, or chemiluminometric method to determine malondialdehyde or 4-hydroxynonenal. Malondialdehyde and 4-hydroxynonenal are derivatives from lipid hydroperoxide prepared by oxidation with reactive oxygen species. Thiobarbituriic acid and 1-Methyl-2-phenylindole are used for deriving malondialdehyde for the colorimetric or fluorometric analysis.

Spy-LHP is a low-fluorescent compound, but is oxidized with lipid hydroperoxide to become a high fluorescent compound as indicated in Fig. 1. A similar product, DPPP, is oxidized by a lipid hydroperoxide and becomes a fluorescence compound that can be excited at 352 nm to emit fluorescence at 380 nm. However, the UV excitation for DPPP significantly damages a live cell. Since the oxidized Spy-LHP emitsstrong fluorescence (quantum yield: ~1) with maximum wavelength at 535 nm when excited at 524 nm, damage to live cells is very small. Spy-LHP has two alkyl chains to improve the affinity to the lipid bilayer. Spy-LHP is highly selective to lipid hydroperoxide and does not react with hydrogen peroxide, hydroxy radicals, superoxide anion, nitric oxides, peroxynitrite, and alkylperoxy radicals.

Fig. 1 Reaction of Spy-LHP with lipid hydroperoxide

1. N. Soh, et al., Novel fluorescent probe for detecting hydroperoxides with strong emission in the visible range. Bioorg Med Chem Lett. 2006;16:2943-2946.2. N. Soh, et al., Swallow-tailed perylene derivative: a new tool for fluorescent imaging of lipid hydroperoxides. Org Biomol Chem. 2007;5:3762-3768.

Related Categories Oxidative Stress Assay

品牌介绍
Dojindo细胞分析细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐,称为WST。WST-8是高度稳定的WST,用于Cell Counting Kit-8(CCK-8)。由于WST-8甲maz是水溶性的,因此不会形成晶体。因此,不需要诸如MTT测定的增溶过程。此外,CCK-8的检测灵敏度高于其他四唑盐,例如MTT,XTT,MTS或WST-1。WST检测机制 ß-半乳糖苷酶检测试剂细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢应用产品展示细胞生长检测,药物筛选,比色/荧光检测细胞计数试剂盒-8细胞计数试剂盒8 + 96孔有机硅定向剂细胞计数试剂盒-F细胞毒性LDH检测试剂盒-WST 96孔有机硅定向剂MTT了解检测机制的差异:点击这里细胞周期分析细胞周期测定溶液深红色细胞周期测定溶液蓝色