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商品详细Dojindo/NBD-F/100/N020
Dojindo/NBD-F/100/N020
Dojindo/NBD-F/100/N020
商品编号: N020
品牌: 同仁
市场价: ¥4780.00
美元价: 2868.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionApplicationReferencesS.D.S

Derivatization Reaction

Product DescriptionNBD-F is highly reactive and can label primary and secondary amines under mild conditions (1 min reaction at 60ºC in a weak basic solution). NBD-F is a pre-labeling compound for HPLC analysis of small molecules. NBD-labeled compounds are orange with a maximum wavelength at 470 nm.The excitation and emission of the derivatized compound are 470 nm and 530 nm, respectively.

NBD Labeling Protocol1. To prepare sample solution, mix or dissolve a sample with 50 mM borate buffer (pH 8.0) containing 20 mM EDTA.2. Mix 300 μl of the sample solution and 100 μl of 100 mM NBD-F/acetonitrile solution in a reaction vial.3. Heat the vial at 60ºC for 1 minute and then cool it on an ice bath.4. Add 400 μl of 50 mM HCl aqueous solution to the reaction mixture.5. Use this mixture for HPLC analysis to determine NBD-labeled compounds.

1. K. Imai, et al., Fluorimetric Determination of Secondary Amino Acids by 7-fluoro-4-nitrobenzo-2-oxa-1, 3-diazole. Anal Chim Acta. 1981;130:377-383.2. Y. Watanabe, et al., High-Performance Liquid Chromatography and Sensitive Detection of Amino Acids Derivatized with 7-fluoro-4-nitrobenzo-2-oxa-1, 3-diazole. Anal Biochem. 1981;116:471-472.3. Y. Watanabe, et al., Pre-column Labelling for High-performance Liquid Chromatography of Amino Acids with 7-fluoro-4-nitrobenzo-2-oxa-1, 3-diazoleand Its Application to Protein Hydrolysates. J Chromatogr A. 1982;239:723-732.4. T. Toyo’oka, et al., Reaction of Amines of Biological Importance with 4-fluoro-7-nitrobenzo-2-oxa-1, 3-diazole. Anal Chim Acta. 1983;149:305-312.5. Y. Watanabe, et al., Liquid Chromatographic Determination of Amino and Imino Acids and Thiols by Postcolumn Derivatization with 4-Fluoro-7-nitrobenzo-2, 1, 3-oxadiazole. Anal Chem. 1983;55:1786-1791.6. Y. Watanabe, et al., Sensitive Detection of Amino Acids in Human Serum and Dried Blood Disc of 3mm Diameter for Diagnosis of Inbornerrors of Metabolism. J Chromatogr. 1984;309:279-286.7. H. Miyano, et al., Further Studies on the Reaction of Amines and Proteins with 4-fluoro-7-nitrobenzo-2-oxa-1, 3-diazole. Anal Chim Acta. 1985;170:81-87.8. H. Kotaniguchi, et al., Automatic Amino Acid Analysis Utilizing 4-fluoro-7-nitrobenzo-2-oxa-1, 3-diazole. J Chromatogr. 1987;420:141-145.9. K. Imai, et al., High-Performance Liquid Chromatography with Photochemical Fluorimetric Detection of Tryptophan Based on 4-fluoro-7-nitrobenzo-2-oxa-1, 3-diazole Total Protein Amino Acid Analysis. Anal Chim Acta. 1988;205:7-14.10. K. Imai, et al., Dynamic Analytical Chemistry: A Trial Study of the Interaction of Fluorogenic Reagents with Living Chinese Hamster Ovary Cells. Anal Chim Acta. 1989;223:299-308.11. C. Nakamura, et al., Dioxin-binding Pentapeptide for Use in a High-sensitivity On-bead Detection Assay. Anal Chem. 2005;77:7750-7757.

Related Categories Labeling Chemistry

品牌介绍
Dojindo细胞分析细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐,称为WST。WST-8是高度稳定的WST,用于Cell Counting Kit-8(CCK-8)。由于WST-8甲maz是水溶性的,因此不会形成晶体。因此,不需要诸如MTT测定的增溶过程。此外,CCK-8的检测灵敏度高于其他四唑盐,例如MTT,XTT,MTS或WST-1。WST检测机制 ß-半乳糖苷酶检测试剂细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢应用产品展示细胞生长检测,药物筛选,比色/荧光检测细胞计数试剂盒-8细胞计数试剂盒8 + 96孔有机硅定向剂细胞计数试剂盒-F细胞毒性LDH检测试剂盒-WST 96孔有机硅定向剂MTT了解检测机制的差异:点击这里细胞周期分析细胞周期测定溶液深红色细胞周期测定溶液蓝色