Fig. 1 Fluorescence spectrum of R-PEExcitation wavelength: 566 nmEmission wavelength: 578 nm
Fig. 2 IgG labeling reaction of NH2-reactive R-PE
Precaution♦ The molecular weight of the protein to be labeled with this kit should be greater than 50,000.♦ IgG or R-phycoerythrin-conjugated IgG is always on the membrane of the filtration tube during the labeling process.♦ If the IgG solution contains other proteins with molecular weight larger than 10,000, such as BSA or gelatin, purify the IgG solution prior to labeling R-phycoerythrin with this kit. IgG solution can be purified by IgG Purification Kits (not included in this kit).♦ If the IgG solution contains small insoluble materials, centrifuge the solution and use the supernatant for the labeling.
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Related Categories Labeling Chemistry