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当前位置: 首页 > 产品中心 > peptide > Dojindo/ExoSparkler Exosome Protein Labeling Kit/Deep/EX04
商品详细Dojindo/ExoSparkler Exosome Protein Labeling Kit/Deep/EX04
Dojindo/ExoSparkler Exosome Protein Labeling Kit/Deep/EX04
Dojindo/ExoSparkler Exosome Protein Labeling Kit/Deep/EX04
商品编号: EX04
品牌: 同仁
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionManualS.D.S(EX04)S.D.S(EX05)S.D.S(EX06)

Product Description

Exosomes are a form of extracellular vesicle (EV), which may contribute to malignant transformation and the metastasis of cancer. Consequently, intercellular communication via exosomes is attracting considerable interest in the scientific community. To shed light on such communication, labeling techniques based on fluorescent dyes have been used. Fluorescent dyes that label the cellular membrane are commonly used for exosome labeling because the lipid bilayer in exosomes is a suitable labeling target. ExoSparkler series can be used for staining of purified exosomal membrane or protein, which allows imaging of labeled exosomes taken up by cells.


Our ExoSparkler Exosome Protein Labeling Kits provide everything from fluorescence labeling to purificationExoSparkler series contains filtration tubes available for the removal of dyes unreacted after fluorescence labeling, as well as an optimized protocol for labeling exosomes. Our ExoSparkler series makes it possible to prepare fluorescence labeling of exosomes using the simple procedure.


ExoSparkler series product comparison

Experimental conditionsExosomes were purified by ultracentrifugation (10μg exosome protein) and stained with each dye. Labeled exosomes were added to HeLa cells (1.25×104 cells), and the cells were incubated for 24 hours. Cells were washed, and immunofluorescence images showing labeled exosomes were observed.Detection conditionsGreen: Ex 488nm/Em 490-540nmRed: Ex 561nm/Em 570-640nmDeep Red: Ex 640nm/Em 640-760nm


Localization of exosomesExosomes stained with ExoSparkler Exosome Protein Labeling Kit-Deep Red(Item#: EX06, Protein Dye-Deep Red)or ExoSparkler Exosome Membrane Labeling Kit-Red(item#:EX02, Mem Dye-Red)were added to HeLa cells, and the cells were co-stained with lysosome staining reagent (Green).

Experimental conditionsExosomes were purified by ultracentrifugation (5μg exosome protein) and stained with each dye. Labeled exosomes were added to HeLa cells (0.75×104 cells), and the cells were incubated for 3.5 hours. After that, cells were stained with lysosome staing reagent (Green). Cells were washed, and immunofluorescence images showing labeled exosomes were observed.

Detection conditionsProtein Dye-Deep Red (purple) : Ex 640nm/Em 640-760nmMemDye-Red (Red) : Ex 561nm/Em 570-640nmLysosome staing reagent (Green). : Ex 488nm/Em 490-540nm

Related Categories Labeling Chemistry

品牌介绍
Dojindo细胞分析细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐,称为WST。WST-8是高度稳定的WST,用于Cell Counting Kit-8(CCK-8)。由于WST-8甲maz是水溶性的,因此不会形成晶体。因此,不需要诸如MTT测定的增溶过程。此外,CCK-8的检测灵敏度高于其他四唑盐,例如MTT,XTT,MTS或WST-1。WST检测机制 ß-半乳糖苷酶检测试剂细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢应用产品展示细胞生长检测,药物筛选,比色/荧光检测细胞计数试剂盒-8细胞计数试剂盒8 + 96孔有机硅定向剂细胞计数试剂盒-F细胞毒性LDH检测试剂盒-WST 96孔有机硅定向剂MTT了解检测机制的差异:点击这里细胞周期分析细胞周期测定溶液深红色细胞周期测定溶液蓝色