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当前位置: 首页 > 产品中心 > peptide > Dojindo/Ab-10 Rapid Peroxidase Labeling Kit/3/LK33
商品详细Dojindo/Ab-10 Rapid Peroxidase Labeling Kit/3/LK33
Dojindo/Ab-10 Rapid Peroxidase Labeling Kit/3/LK33
Dojindo/Ab-10 Rapid Peroxidase Labeling Kit/3/LK33
商品编号: LK33
品牌: 同仁
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionApplicationManualS.D.S

Product DescriptionAb-10 Rapid Peroxidase Labeling Kit is for labeling peroxidase to 10 µg antibody in less than 30 minutes. Labeling agent included in the kit has succinimidyl ester group , which can easily make a covalent bond with an amino group of the target antibody without any activation process. The kit contains all the necessary reagents for antibody labeling.

Fig. 1 IgG Labeling Reaction of NH2-reactive Peroxidase

Fig. 2 Labeing Procedure

Precaution♦ Use 0.5-1 mg/ml of antibody solution for labeling. If the antibody concentration is higher than 1mg/ml, dilute the antibody solution with PBS.♦ If the sample solution contains small insoluble materials, centrifuge the solution, and use the supernatant for the labeling.♦ The microtube in this kit contain solutions. Since there is a possibility that the droplets might be attached to the inside walls or caps, please centrifuge to drop the droplets prior to opening.♦ Some additives in antibody solution may interfere with the labeling if the concentration is too high. The maximum compatible concentrations of such additives are indicated on Table 1.♦ After a Reactive Fluorescent is removed from the seal bag, keep the unused Reactive Fluorescein in the bag, seal tightly and store at -20ºC.Store the other components at 0-5ºC.♦ Since reactive reagent binds to an amino group in antibody, there is a possibility that the labeled antibody loses the antigen recognition ability (Table 2).

Table. 1 Compatible concentrations of the additives

Table. 2 Non-Compatible Antibody

Experimental Data

Fig. 3 Microscope Image of Mitochondria in Hela CellsPeroxidase were to anti-mitochondria antibody using Ab-10 Rapid Peroxidase Labeling Kit (code:LK33)

Related Categories Labeling Chemistry Intracellular Fluorescent Probes

品牌介绍
Dojindo细胞分析细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐,称为WST。WST-8是高度稳定的WST,用于Cell Counting Kit-8(CCK-8)。由于WST-8甲maz是水溶性的,因此不会形成晶体。因此,不需要诸如MTT测定的增溶过程。此外,CCK-8的检测灵敏度高于其他四唑盐,例如MTT,XTT,MTS或WST-1。WST检测机制 ß-半乳糖苷酶检测试剂细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢应用产品展示细胞生长检测,药物筛选,比色/荧光检测细胞计数试剂盒-8细胞计数试剂盒8 + 96孔有机硅定向剂细胞计数试剂盒-F细胞毒性LDH检测试剂盒-WST 96孔有机硅定向剂MTT了解检测机制的差异:点击这里细胞周期分析细胞周期测定溶液深红色细胞周期测定溶液蓝色