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商品详细Dojindo/EMCS/100/E018
Dojindo/EMCS/100/E018
Dojindo/EMCS/100/E018
商品编号: E018
品牌: 同仁
市场价: ¥4120.00
美元价: 2472.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionApplicationReferencesS.D.S
Cross-linking Reaction

Product DescriptionHetero-bifunctional cross-linking reagents have activated esters and maleimide reactive groups. These functional groups react with amines and sulfhydryl groups of proteins, respectively. Enzyme-labeled haptens are prepared using hetero-bifunctional cross-linking reagents such as EMCS or GMBS. The cross-linking reaction requires neutral pH and mild temperature because it is necessary to maintain enzyme activity and antibody titers in the cross-linking reaction. Hetero-bifunctional cross-linking reagents with 3, 5, 7, or 10 linear carbon chains are available. These linear aliphatic chains act as spacers between the two reactive sites and their water-soluble reagents. They are more stable than the aromatic cross-linking reagents such as succinimidyl-4-N-maleimidobenzoate in a wider pH range.

Conjugation of Macromolecules with Hetero-Bifunctional Cross-Linking Reagent

Hetero-bifunctional Reagents

Product NameCodeLength (Å)Solvent
EMCSE0189.4DMSO
GMBSG0056.9Chloroform, DMF
HMCSH25713.0Acetonitrile
KMUSK21416.7Acetonitrile, Methyl alcohol

 

1. S. Yoshitake, Y. Yamada, E. Ishikawa and R. Masseyeff, Conjugation of Glucose Oxidase from Aspergillus Niger and Rabbit Antibodies Using N-Hydroxysuccinimide Ester of N-(4-Carboxycyclohexylmethyl)-Maleimide, Eur. J. Biochem., 1979, 101, 395.2. T. Kitagawa, T. Shimozono, T. Aikawa, T. Yoshida and H. Nishimura, Preparation and Characterization of Hetero-bifunctional Cross-linking Reagents for Protein Modifications, Chem. Pharm. Bull., 1981, 29, 1130.3. S. Yoshitake, M. Imagawa, E. Ishikawa, Y. Niitsu, I. Urushizaki, M. Nishimura, R. Kanazawa, H. Kurosaki, S. Tachibana, N. Nakazawa and H. Ogawa, Mild and Efficient Conjugation of Rabbit Fab Eand Horseradish Peroxidase Using a Maleimide Compound and Its Use for Enzyme Immunoassay, J. Biochem., 1982, 92, 1413.4. E. Ishikawa, M. Imagawa, S. Hashida, S. Yoshitake, Y. Hamaguchi and T. Ueno, Enzyme-Labelling of Antibodies and Their Fragments for Enzyme Immunoassay and Immunohistochemical Staining, J. Immunol., 1983, 4, 209.5. I. Weeks, A. K. Campbell and J. S. Woodhead, Two-site Immunochemiluminometric Assay for Human α1-Fetoprotein, Clin. Chem., 1983, 29, 1480.6. S. Hashida, M. Imagawa, S. Inoue, K. Ruan and E. Ishikawa, More Useful Maleimide Compounds for the Conjugation of Fab Eto Horseradish Peroxidase through Thiol Groups in the Hinge, J. Appl. Biochem., 1984, 6, 56.7. S. Inoue, M. Imagawa, S. Hashida, K. Ruan and E. Ishikawa, A Small Scale Preparation of Affinity-Purified Rabbit Fab EHorseradish Peroxidase Conjugate for Enzyme Immunoassay, Anal. lett., 1984, 17, 229.8. M. Koizumi, K. Endo, M. Kunimatsu, H. Sakahara, T. Nakashima, Y. Kawamura, Y. Watanabe, T. Saga, J. Konishi, T. Yamamura, S. Hosoi, S. Toyama, Y. Arano and A. Yokoyama, Ga-Labeled Antibodies for Immunoscintigraphy and Evalution of Tumor Targeting of Drug-Antibody Conjugatesin Mice, Cancer Res., 1988, 48, 1189.9. T. Kohno and E. Ishikawa, Novel Enzyme Immunoassay(Immune Complex Transfer Enzyme Immunoassay) for Anti-insulin IgG in Guinea Pig Serum, Anal. lett., 1988, 21, 1019.

Related Categories Surface Chemistry

品牌介绍
Dojindo细胞分析细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐,称为WST。WST-8是高度稳定的WST,用于Cell Counting Kit-8(CCK-8)。由于WST-8甲maz是水溶性的,因此不会形成晶体。因此,不需要诸如MTT测定的增溶过程。此外,CCK-8的检测灵敏度高于其他四唑盐,例如MTT,XTT,MTS或WST-1。WST检测机制 ß-半乳糖苷酶检测试剂细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢应用产品展示细胞生长检测,药物筛选,比色/荧光检测细胞计数试剂盒-8细胞计数试剂盒8 + 96孔有机硅定向剂细胞计数试剂盒-F细胞毒性LDH检测试剂盒-WST 96孔有机硅定向剂MTT了解检测机制的差异:点击这里细胞周期分析细胞周期测定溶液深红色细胞周期测定溶液蓝色