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当前位置: 首页 > 产品中心 > peptide > Dojindo/Biotin-PEAC5-maleimide/10/B299
商品详细Dojindo/Biotin-PEAC5-maleimide/10/B299
Dojindo/Biotin-PEAC5-maleimide/10/B299
Dojindo/Biotin-PEAC5-maleimide/10/B299
商品编号: B299
品牌: 同仁
市场价: ¥0.00
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产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
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商品介绍
DescriptionApplicationReferencesS.D.S

Product Description of Amine-Reactive BiotinsThe avidin-biotin system has many applications in immunology and histochemistry. The interaction between avidin and biotin is remarkably strong with a dissociation constant on the order of the 10-15 M. Biotin is usually added to primary or secondary antibodies such as anti-IgG and anti-IgM. After preparing the antigen-antibody complex with the biotin-labeled antibody, colorimetric or fluorometric detection of the antigen is performed using enzyme or fluorophore-labeled avidin or streptavidin. Maleimide biotins react with thiol compounds, such as proteins or peptides with sulfhydryl groups, at pH 7-7.5. Maleimide reacts with sulfhydryl group to create a thioether bond. Though other maleimide biotin reagents must be dissolved in DMSO, DMF, or alcohol, Biotin-PE-maleimide can be solubilized in PBS at pH 7.4 to prepare 2 mM solution without using an organic solvent. The reactivity of maleimide with sulfhydryl groups is higher than that of bromoacetamide, so the required concentration of maleimide biotin is much lower than that of bromoacetamide biotins. Stock solutions of Biotin-PE-maleimide and Biotin-PEAC5-maleimide in DMSO are stable for one year at -20oC.

Structural Formula:

Labeling Procedure for Reduced IgG1. Prepare 10 mM of the biotin labeling reagent using DMSO.2. Prepare 100 μl of 1 mg per ml reduced IgG/ml buffer solution which does not contain any large molecules with SH groups. Reduced IgG can be prepared by TCEP (tricarboxyethylphosphine), DTT or 2-mercaptoethylamine.3. Add 1-5 μl of biotin labeling reagent DMSO solution to the IgG buffer solution and incubate at 37 oC for 1 hour.4. Remove excess biotin labeling reagent using a gel column or a Filtration tube.5. Prepare solutions for further experiment using an appropriate buffer such as PBST (0.05% Tween 20/PBS).

E. Muneyuki, et al., Biophys. J., 92, 1806 (2007).

Related Categories Labeling Chemistry

品牌介绍
Dojindo细胞分析细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐,称为WST。WST-8是高度稳定的WST,用于Cell Counting Kit-8(CCK-8)。由于WST-8甲maz是水溶性的,因此不会形成晶体。因此,不需要诸如MTT测定的增溶过程。此外,CCK-8的检测灵敏度高于其他四唑盐,例如MTT,XTT,MTS或WST-1。WST检测机制 ß-半乳糖苷酶检测试剂细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢应用产品展示细胞生长检测,药物筛选,比色/荧光检测细胞计数试剂盒-8细胞计数试剂盒8 + 96孔有机硅定向剂细胞计数试剂盒-F细胞毒性LDH检测试剂盒-WST 96孔有机硅定向剂MTT了解检测机制的差异:点击这里细胞周期分析细胞周期测定溶液深红色细胞周期测定溶液蓝色