4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:主营:化学试剂,CCK-8试剂盒,细胞检测,自噬研究,化学标记,生化试剂等
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > peptide > Dojindo/ARP(醛反应探针)/25/A305
商品详细Dojindo/ARP(醛反应探针)/25/A305
Dojindo/ARP(醛反应探针)/25/A305
Dojindo/ARP(醛反应探针)/25/A305
商品编号: A305
品牌: 同仁
市场价: ¥8520.00
美元价: 5112.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionApplicationReferencesS.D.S

Product DescriptionAldehyde reactive probe (ARP) is used to detect abasic sites (AP sites, depurine/depyrimidine sites) in DNA because of its aldehyde-specific reactivity. ARP reacts with aldehyde and ketone groups, and it adds biotin to AP sites in DNA. The biotin-tagged DNA is then detected using peroxidase-labeled avidin and oxidative chromogenic dyes. The ARP method has been used to detect less than 1 AP site in 1×104 nucleotides of DNA. Dojindo offers a DNA Damage Quantification Kit (Product Code: DK02) containing all the reagents and components necessary to determine 1 to 40 ARP sites per 1×105 base pairs. ARP is highly soluble in water and the stock solution can be stored at 4ºC for one year without significant loss of reactivity.

Reaction Scheme

Labeling Procedure for DNA Abasic Site1. Prepare 10 mM ARP with water.2. Adjust the DNA concentration to 100 μg per ml with TE buffer (pH 7.4).3. Mix equal volumes of the DNA and ARP solutions and incubate at 37ºC for 1 hour.4. Isolate the ARP-labeled DNA using either ethanol precipitation or membrane filtration tube.5. Dissolve the DNA pellet in TE to prepare 10-100 μg per ml solution.

1. R. Rago, J. Mitchen and G. Wilding, DNA Fluorometric Assay in 96-well Tissue Culture Plates Using Hoechst 33258 after Cell Lysis by Freezing in Distilled Water, Anal. Biochem., 1990, 191, 31.2. K. Kubo, H. Ide, S. S. Wallace and Y. W. Kow, A Novel, Sensitive, and Specific Assay for Abasic Sites, The Most Commonly Produced DNA Lesion, Biochemistry, 1992, 31, 3703.3. H. Ide, K. Akamatsu, Y. Kimura, K. Michiue, K. Makino, A. Asaeda, Y. Takamori and K. Kubo, Synthesis and Damage Specificity of a Novel Probe for the Detection of Abasic Sites in DNA, Biochemistry, 1993, 32, 8276.4. M. Yao and Y. W. Kow, Strand-specific Cleavage of Mismatch-containig DNA by Deoxyinosine 3 EEndonuclease from Escherichia coli, J. Biol. Chem., 1994, 269, 31390-6.5. T. Shida, M. Noda and J. Sekiguchi, The recognition of DNA containning AP-site by E. coli endonuclease IV(exonuclease), Nucleic Acids Symposium Series, 1995, 34, 87.6. H. B. Sun, L. Qian and H. Yokota, Detection of abasic sites on individual DNA molecules using atomic force microscopy, Anal. Chem., 2001, 73, 2229.7. J. Nakamura, J. A. Swenberg, Endogenous Apurinic/apyrimidinic Sites in Genomic DNA of Mammalian Tissues, Cancer Res., 1999, 59, 2522-2526.8. Y. W. Kow and A. Dare, Detection of Abasic Sites and Oxidative DNA Base Damage using an ELISA-like Assay, METHODS, 2000, 22, 164.9. L. Yan, A. Bulgar, Y. Miao, V. Mahajan, J. R. Donze, S. L. Gerson and L. Liu, Combined Treatment with Temozolomide and Methoxyamine: Blocking Apurininc/Pyrimidinic Site Repair Coupled with Targeting Topoisomerase II, Clin. Cancer Res., 2007, 13, 1532.10. P. D. Chastain, II, J. Nakamura, J. Swenberg and D. Kaufman, Nonrandom AP site distribution in highly proliferative cells, FASEB J., 2006, 20, 2612.

Related Categories Oxidative Stress Array

品牌介绍
Dojindo细胞分析细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐,称为WST。WST-8是高度稳定的WST,用于Cell Counting Kit-8(CCK-8)。由于WST-8甲maz是水溶性的,因此不会形成晶体。因此,不需要诸如MTT测定的增溶过程。此外,CCK-8的检测灵敏度高于其他四唑盐,例如MTT,XTT,MTS或WST-1。WST检测机制 ß-半乳糖苷酶检测试剂细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢应用产品展示细胞生长检测,药物筛选,比色/荧光检测细胞计数试剂盒-8细胞计数试剂盒8 + 96孔有机硅定向剂细胞计数试剂盒-F细胞毒性LDH检测试剂盒-WST 96孔有机硅定向剂MTT了解检测机制的差异:点击这里细胞周期分析细胞周期测定溶液深红色细胞周期测定溶液蓝色