What is SAM Formation Reagent?SAM (Self-Assembled Monolayer) is a highly organized thin layer of molecules formed on a solid surface by chemisorption. Due to its high applicability, SAM can be used on biosensors. However, unspecific binding is commonly observed while biomaterial samples are used. In the most of these cases, the concentration and combination of SAM reagents are not optimized for preparing an organized layer on a metal surface. Dojindo provides SAM Formation Reagents which are already optimized in one bottle and simple to use. The optimized reagent can reduce unspecific binding significantly.

Comparison DataDojindo SAM Formation Reagent gives us lower background and higher S/N ratio.

Data by QCM

Experimental Conditions:His-tagged ProteinA : 10 ug/ml His-tagged ProteinA in bufferBuffer : 10 mM HEPES (pH 7.2) ,150 mM NaCl , 0.5%Tween-20IgG : 5.5 ug/ml Rabbit IgG in buffer

Instrument： Affinix QNμ(QCM2008-LVKIT), INITIUM, Inc.

Leo JC, Oberhettinger P, Yoshimoto S, Udatha DB, Morth JP, Schutz M, Hori K, Linke D., “Secretion of the Intimin Passenger Domain is driven by Protein Folding.”, Journal of Biological Chemistry ., 2016, doi: 10.1074/jbc.M116.731497