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商品详细Dojindo/IgG Purification Kit-G/1/AP02
Dojindo/IgG Purification Kit-G/1/AP02
Dojindo/IgG Purification Kit-G/1/AP02
商品编号: AP02
品牌: 同仁
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionDataQ & AManualS.D.S

Product DescriptionIgG Purification Kits are used for isolation and purification of immunoglobulin G of goat, mouse, rabbit, and other animals. The kit contains immobilized protein A or G and buffer solutions for maximum recovery of IgG. The total time to isolate and purify IgG from serum or other solutions containing IgG is about 30 min (Fig. 1). Since the support of protein A or G is silica-base gel, the volume of the retained solution on the gel after the centrifugation is very small. Therefore, all proteins or other materials that do not bind to protein A can be removed by washing twice. SDS-PAGE of purified IgG from various animals are shown in Fig. 2. Additionally, denaturing of IgG during the elution process is minimal because IgGs bound to the gel are released with a very quick process. The gel in this kit can be used repeatedly 20 times or more with an equal performance. Used gel is also stable for one year in Washing buffer at 0-5ºC .

Fig. 1 IgG isolation process

Fig. 2 SDS-PAGE of isolated immunoglobulin by using IgG PurificationPurification Kit-A and Kit-G.WS : Whole SerumA : Prepared by IgG Purification Kit-AG : Prepared by IgG Purification Kit-GCondition: 6% acrylamide gel/Tris-glucine buffer

PrecautionIf the IgG solution contains gelatin,before applying the solution to the kit, enzyme digestion may be required.

Table 1 IgG Recovery from 50 μl Serum

What is the recovery of IgG with this kit?

It depends on the type of IgG and type of animal. In the case of high affinity type IgG for protein A or protein G, about 70-80% IgG is recovered from 100-200 μg IgG or IgG solution containing other proteins or macromolecules.

How is the purity of purified IgG using this kit?

The purity of the IgG from various serum is indicated in the figure above. Highly purified IgG is available with only a one time purification process.

How much IgG can be recovered from serum?

About 150-350 μg IgG can be recovered from 50 μl serum. I-7. Protein Labeling: IgG purification

How many times can a protein A gel be used?

At least 20 times.

Can I use the used protein A gel or protein G gel for the purification of different IgG solutions?

Use a new gel for a different sample to avoid contamination.

Is a used protein A gel or protein G gel stable?

A used protein A gel or protein G gel in Washing buffer is stable at 0-5ºC for one year.

Related Categories Labeling Chemistry

品牌介绍
Dojindo细胞分析细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐,称为WST。WST-8是高度稳定的WST,用于Cell Counting Kit-8(CCK-8)。由于WST-8甲maz是水溶性的,因此不会形成晶体。因此,不需要诸如MTT测定的增溶过程。此外,CCK-8的检测灵敏度高于其他四唑盐,例如MTT,XTT,MTS或WST-1。WST检测机制 ß-半乳糖苷酶检测试剂细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢应用产品展示细胞生长检测,药物筛选,比色/荧光检测细胞计数试剂盒-8细胞计数试剂盒8 + 96孔有机硅定向剂细胞计数试剂盒-F细胞毒性LDH检测试剂盒-WST 96孔有机硅定向剂MTT了解检测机制的差异:点击这里细胞周期分析细胞周期测定溶液深红色细胞周期测定溶液蓝色