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商品详细Dojindo/ES/iPS差异化监测工具包/for/ES01
Dojindo/ES/iPS差异化监测工具包/for/ES01
Dojindo/ES/iPS差异化监测工具包/for/ES01
商品编号: ES01
品牌: 同仁
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionDataManualS.D.S

Product Description :One of the secretory proteins as in a culture medium was found to be a marker of the conversion level of ES and iPS to endodermal cells. (Published in: H. Iwashita, S. Kume, PloS ONE., 2013, 8(5): e64291)The amount of this marker protein in the cell culture supernatant is determined by ELISA. It can be used to monitor the efficiency of differentiation of endodermal cells from ES/iPS cells. Since this kit is designed for the 96-well microplate format, it is suitable for multiple sample measurements, such as screening of inducers for differentiation or an optimization of culture conditions.

This product was developed by a collaborative work between Institute of Molecular Embryology and Genetics, Kumamoto University and Dojindo Laboratories.


RT-PCR Measurement of each Marker :The secretory protein has a good correlation with Sox 17 and Foxa2 which are commonly used proteins as markers of endoderm differentiation.


Comparison with Conventional Techniques :

Dojindo KitPCRImmunostainingFlow Cytometry
Procedure3 hours> 7 hours1-2 days> 4 hours
SampleSupernatantmRNAWhole cellsWhole cells
ContinuousCell CulturePossibleImpossibleImpossibleImpossible
MarkerMarker ProteinSox17, Foxa2Sox17, Foxa2CXCR4

Measurement of Procedure :

mmunocytochemical(ICC) Analysis:[Sample: Murine ES cell]

Most of the Foxa2 positive cells are co-stained with the marker protein.The three images in the upper row were obtained from conventional ICC analysis. Normally, Foxa2 and Sox17 double positive cells are defined as differentiated into endodermal cells.


Differentiation Monitoring by ICC and ELISA:[Sample: Human iPS cell]

Human iPS 253G1 cells were differentiated into the definitive endoderm. They were being monitored with ELISA assay to measure the amount of marker protein secreted or ICC analysis for Sox17 and Foxa2 double positive cells.

The two bar graphs show the correlation of the amount of secreted protein with the amount of Sox17 and Foxa2 double positive cells from day 1 to day 5 (D1 to D5) of differentiation.

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