4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:主营:化学试剂,CCK-8试剂盒,细胞检测,自噬研究,化学标记,生化试剂等
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > peptide > Dojindo/抗硝基鸟苷多克隆抗体/50/AB01
商品详细Dojindo/抗硝基鸟苷多克隆抗体/50/AB01
Dojindo/抗硝基鸟苷多克隆抗体/50/AB01
Dojindo/抗硝基鸟苷多克隆抗体/50/AB01
商品编号: AB01
品牌: 同仁
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionReferencesS.D.S

Product Description8-Nitroguanosine is a nitrated base of DNA and RNA. It is formed by peroxynitrite, which is generated from nitric oxide and superoxide anion radical. It is known that a large amount of nitric oxide molecules and superoxide anion, generated by inflammation, causes nitration of guanosine. Since chemically modified nucleotides cause mutation during DNA replication, 8-nitroguanosine is thought to be one of the markers of DNA damage related to mutation and cancer. Because of its very high specificity, monoclonal antibody NO2G52 recognizes 8-nitroguanine and 8-nitroguanosine, but it does not cross-react with normal nucleotide bases, 8-hydroxyguanine, 8-hydroxydeoxyguanosine, 3-nitrotyrosine, xanthine, or 2-nitroimidazole (Fig. 1). The specificity of NO2G52 was determined by a competitive ELISA using an 8-nitroguanosine-BSA-coated plate. As shown in the figures below, NO2G52 has very high affinity for 8-nitroguanine and 8-nitroguanosine, and it slightly cross-reacts with 8-bromoguanosine, 8-bromoguanine, and 8-chloroguanine. Anti-Nitroguanosine polyclonal antibody also recognizes 8-nitroguanine and 8-nitroguanosine, but it does not cross-react with normal guanosine, guanine, 8-hydroxyguanine, or 3-nitrotyrosine. Since this antibody was prepared using rabbits, it can be used for immuno-histostaining of rodent tissues such as mice or rats.

Fig 1. Specificity of Anti 8-Nitroguanosine monoclonal antibody

Fig 2 Tissue Staining with Anti-Nitroguanosine AntibodyA) Tissue section (8 days postinfection, influenza) stained with Anti-Nitroguanosine Antibody.B) The same section viewed using a confocal laser scanning microscope (Fluoroview FV300, Olympus, Nagano, Japan). Strong fluorescence, due to emission of Vector red, is evident in the cytosol.

1. T. Akaike, et al., 8-nitroguanosine formation in viral pneumonia and its implication for pathogenesis, PNAS. 2003;100:685-690.2. J. Yoshitake, et al., Nitric oxide as an endogenous mutagen for Sendai virus without antiviral activity. J Virol. 2004;78:8709-8719.3. T. Sawa, et al., Protein S-guanylation by the biological signal 8-nitroguanosine 3 E5 Ecyclic monophosphate. Nat Chem Biol. 2007;3:727-735.4. M. H. Zaki, et al., Cytoprotective function of heme oxygenase 1 induced by a nitrated cyclic nucleotide formed during murine salmonellosis. J Immunol. 2009;182:3746-3756.5. Y. Terasaki, et al., Guanine nitration in idiopathic pulmonary fibrosis and its implication for carcinogenesis. Am J Respir Crit Care Med. 2006;174:665-673.6. T. Sawa, et al., Analysis of urinary 8-nitroguanine, a marker of nitrative nucleic acid damage, by high-performance liquid chromatography-electrochemical detection coupled with immunoaffinity purification: association with cigarette smoking. Free Radic Biol Med. 2006;40:711-720.

品牌介绍
Dojindo细胞分析细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐,称为WST。WST-8是高度稳定的WST,用于Cell Counting Kit-8(CCK-8)。由于WST-8甲maz是水溶性的,因此不会形成晶体。因此,不需要诸如MTT测定的增溶过程。此外,CCK-8的检测灵敏度高于其他四唑盐,例如MTT,XTT,MTS或WST-1。WST检测机制 ß-半乳糖苷酶检测试剂细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢应用产品展示细胞生长检测,药物筛选,比色/荧光检测细胞计数试剂盒-8细胞计数试剂盒8 + 96孔有机硅定向剂细胞计数试剂盒-F细胞毒性LDH检测试剂盒-WST 96孔有机硅定向剂MTT了解检测机制的差异:点击这里细胞周期分析细胞周期测定溶液深红色细胞周期测定溶液蓝色