4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:主营:化学试剂,CCK-8试剂盒,细胞检测,自噬研究,化学标记,生化试剂等
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > peptide > Dojindo/Biofilm Formation Assay Kit/100/B601
商品详细Dojindo/Biofilm Formation Assay Kit/100/B601
Dojindo/Biofilm Formation Assay Kit/100/B601
Dojindo/Biofilm Formation Assay Kit/100/B601
商品编号: B601
品牌: 同仁
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionMovieQ & AManualS.D.S

Biofilms are biological aggregates consisting of microorganisms and extracellular polysaccharides that can exist in many environments. The microorganisms in biofilms are highly resistant to antibiotics; therefore, research into medicines and food ingredients that have anti-biofilm activities is a growing area.Our assay kits for the quantitative measurements of biofilm formation/inhibition of biofilm formation (Biofilm Formation Assay Kit) and anti-biofilm drug efficacy (Biofilm Viability Assay Kit) adopted a protocol which is designed to use the peg plate from biofilm formation. Our assay kits combined all the necessary components into each package.

Feature 1: Measurement time can be significantly shortened.In existing methods, biofilms are formed on the bottom of the well of a microplate, and additional work is required to change microbial culture media and wash sample several times before and after staining. However, our assay kits allow you to form biofilms on the pegs attached to the plate’s lid. Media change and staining process can be done simply by transferring the lid; therefore, our assay kits are very easy to use.

Feature 2: Our assay kits can reduce measurement variation.In existing methods, biofilm formation occurs on the bottom of the well of a microplate, which made biofilm easier to peel off because of the processes such as washing. This causes measurement variation, and this measurement variation was an issue for the quantification of biofilm. Our assay kits allow you to form biofilms on the pegs attached to the plate’s lid and make it possible to avoid peeling off biofilm which occurs during the process of the formation.

Two different types of assay kits for different purposes.We offer two types of assay kits that measure the amount of biofilm formation or the metabolic activities of live microorganisms within biofilms by using the same measurement method. You can select the one that best suits your needs.

Selection of 2 types of assay kits

*Conditions on biofilm formation vary according to microbial species and strains. When you need to examine these conditions, we first recommend you use the Biofilm Formation Assay Kit.*The Biofilm Formation Assay Kit is a product developed together with Fukuoka Industrial Technology Center.

Procedure

Example of measurementIndicators for drug-mediated inhibition of biofilm formation or antimicrobial effect of biofilms include MBIC (minimum biofilm inhibitory concentrations) and MBEC (minimum biofilm eradication concentrations). MBIC and MBEC of S. aureus were measured using each kit.

[How to use – Quick Tutorial]

Q1. How can I examine the conditions on biofilm formation?
A1. Test items for conditions on biofilm formation include types of culture media, seeding density of microorganisms, frequency of media changes, incubation time, incubation temperature, and others.We show examples of optimizing the following test items: types of culture media, seeding density of microorganisms, frequency of media changes, and incubation time, as below.If you optimize incubation temperature, it is necessary to use a number of the Biofilm Formation Assay Kits (Product code: B601).

Measure the amount of biofilm formation – ①Under the following optimized conditions: types of culture media, seeding density of microorganisms, and media changes, the amount of biofilm formation can be confirmed by the experiments shown below.

Example of plate arrangement

Experimental schedule

Procedure(Step 1) Day 1: 96-well plate ①In accordance with the reference layout shown above, fill each well (rows A and B) of the 96-well plate with 180μl of microbial cell suspension with concentrations ① and ② made in culture media A through D.Leave the wells (rows C through H) blank. Place a 96-peg lid on the 96-well plate and incubate the plate at the optimum growth temperature for the microorganism for 24 hours.※ ExamplesMicrobial concentrations ① and ②: Approximately 107 CFU/ml and 106 CFU/ml, respectively.(Step 2) Day 2: 96-well ②In accordance with the reference layout shown above, fill each well (rows A and B) of the 96-well plate with 180μl of culture media without microorganism.Fill each well (rows C and D) of the same 96-well plate with 180μl of microbial cell suspension with concentrations ① and ② made in culture media A through D. Leave the wells (rows E through H) blank.Place the 96-peg lid (Step1) on the 96-well plate ② and incubate the 96-well plate ② at the optimum growth temperature for the microorganism for 24 hours.(Step 3) Day 3: 96-well plate ③In accordance with the reference layout shown above, fill each well (rows A through D) of the 96-well plate with 180μl of culture media without microorganism.Fill each well (rows E and F) of the same 96-well plate with 180 μl of microbial cell suspension with concentrations ① and ② made in culture media A through D. Leave the wells (rows G and H) blank.Place the 96-peg lid (Step1) on the 96-well plate ③ and incubate the 96-well plate at the optimum growth temperature for the microorganism for 24 hours.(Step 4) Day 4: 96-well plate ④In accordance with the reference layout shown above, fill each well (rows A through F) of the 96-well plate with 180μl of culture media without microorganism.Fill each well (rows G and H) of the same 96-well plate with 180μl of microbial cell suspension with concentrations ① and ② made in culture media A through D.Place the 96-peg lid (Step1) on the 96-well plate ④ and incubate the 96-well plate ④ at the optimum growth temperature for the microorganism for 24 hours.(Step5) Perform experiments using a new 96-well plate, in accordance with Steps 2 through 6 which describe the measurements of biofilm formation/inhibition of biofilm formation in the technical manual.Measure the amount of biofilm formation – ②Under the following optimized conditions: types of culture media, seeding density of microorganisms, and media changes, the amount of biofilm formation can be confirmed by the experiments shown below.

Example of plate arrangement

Experimental schedule

Procedure(Step 1) Day 1: 96-well plate○1In accordance with the reference layout shown above, fill each well (rows A and B) of the 96-well plate with 180μl of microbial cell suspension with concentrations ① and ② made in culture media A through D.Leave the wells (rows C through H) blank.Place a 96-peg lid on the 96-well plate and incubate the plate at the optimum growth temperature for the microorganism for 24 hours.※ ExamplesMicrobial concentrations ① and ②: Approximately 107 CFU/ml and 106 CFU/ml, respectively.(Step2) Day 2: Remove the 96-peg lid. Allow cells to grow in the rows A and B of the 96-well plate○1 without changing the culture medium.Fill each well (rows C and D) of the same 96-well plate with 180μl of microbial cell suspension with concentrations ① and ② made in culture media A through D. Leave the wells (rows E through H) blank.Place the 96-peg lid back to the 96-well plate ①. Continuously incubate the 96-well plate ① at the optimum growth temperature for the microorganism for 24 hours.(Step3) Day 3: Remove the 96-peg lid. Allow cells to grow in the rows (A through D) of the 96-well plate ①without changing the culture medium.Fill each well (rows E and F) of the same 96-well plate with 180μl of microbial cell suspension with concentrations ① and ② made in culture media A through D. Leave the wells (rows G and H) blank. Place the 96-peg lid back to the 96-well plate ①. Continuously incubate the 96-well plate ① at the optimum growth temperature for the microorganism for 24 hours.(Step 4) Day 4: Remove the 96-peg lid. Allow cells to grow in the rows (A through F) of the 96-well plate ① without changing the culture medium.Fill each well (rows G and H) of the same 96-well plate with 180 μl of microbial cell suspension with concentrations ① and ② made in culture media A through D. Leave the wells (rows G and H) blank. Place the 96-peg lid back to the 96-well plate ①. Continuously incubate the 96-well plate ① at the optimum growth temperature for the microorganism for 24 hours.(Step 5): Perform experiments using a new 96-well plate, in accordance with Steps 2 through 6 which describe the measurements of biofilm formation/inhibition of biofilm formation in the technical manual.

Q2. What types of microbial species have been tested?
A2. Dojindo Laboratories has evaluated the following microbial species that formed biofilms using the Biofilm Formation Assay Kit (this product) and the Biofilm Viability Assay Kit (Product code: B603).– Staphylococcus aureus– Pseudomonas aeruginosa– Escherichia coli– Streptococcus mutans– Porphyromonas gingivalis

Please refer to the technical manual, in terms of culture conditions for the above microbial species, experimental examples, etc.

Q3. When I evaluate inhibition of biofilm formation and anti-biofilm drug efficacy, what is the approximate amount of biofilm formation required for experiment?

A3. In the Biofilm Formation Assay Kit (Product code: B601), please consider the amount of biofilm formation as a rough indication when a value of absorbance (590 nm) for crystal violet solution obtained after the final step is greater than 0.5.

Related Categories Microbial Viability Assay SOD Activity, Glutathione, DNA Damage

品牌介绍
Dojindo细胞分析细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐,称为WST。WST-8是高度稳定的WST,用于Cell Counting Kit-8(CCK-8)。由于WST-8甲maz是水溶性的,因此不会形成晶体。因此,不需要诸如MTT测定的增溶过程。此外,CCK-8的检测灵敏度高于其他四唑盐,例如MTT,XTT,MTS或WST-1。WST检测机制 ß-半乳糖苷酶检测试剂细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢应用产品展示细胞生长检测,药物筛选,比色/荧光检测细胞计数试剂盒-8细胞计数试剂盒8 + 96孔有机硅定向剂细胞计数试剂盒-F细胞毒性LDH检测试剂盒-WST 96孔有机硅定向剂MTT了解检测机制的差异:点击这里细胞周期分析细胞周期测定溶液深红色细胞周期测定溶液蓝色