PrincipleThis kit can detect glucose found within cell culture medium or intracellular glucose by measuring the absorbance of the colored WST formazan dye. The degree of absorbance depends on the amount of glucose present within the sample.This kit contains a glucose standard solution that can be used to create a standard curve. This allows for the quantitation of glucose levels present in the sample.

ProcedureProcedures are so easy that you simply incubate the plate after the addition of culture supernatant or tissue/cell lysates prior to adding a reagent.

Preparation of Standard CurveGlucose levels in a sample can be measured by a calibration curve established with Glucose Standards included in this kit. If the glucose levels are greater than or equal to 0.5 mmol/L, the sample must be diluted before measurement.

Measurement of Glucose level in combination with Lactate Assay KitBy using Glucose Assay Kit-WST and Lactate Assay Kit-WST, we have successfully measured metabolic activity changes of phloretin, a protein transport inhibitor, when it is added to Jurkat cells.

Precautions when using this kit.

Cell counts may need to be normalized. When cells are analyzed in a microplate, the results obtained may sometimes differ depending on cell numbers per well.In such cases, normalization of the measured values obtained from cell counting and total protein will be necessary.In this kit, cell numbers can be easily measured by the fluorescence intensity induced by a reagent added to cell culture medium for staining nuclei.