Product DescriptionHoechst dyes are cell membrane permeable and stain DNA to emit intense blue fluorescence. They bind to DNA in the minor groove of poly-AT sequence rich areas. Both Hoechst 33342 and Hoechst 33258 are water-soluble and stable in aqueous solutions. The excitation and emission wavelengths of Hoechst-DNA complex are 350 nm and 460 nm, respectively.
Chemical Structure
a) Since Hoechst dyes may be carcinogenic, extreme care is necessary during handling.b) Or you may replace the culture medium with 1/10 concentration of Hoechst dye buffer solution.
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Staining Data
Fig. 2 Cell staining with Hoechst 33258Cell type: human fetal cell
Fig. 3 Cell staining with Hoechst 33342Cell type: human fetal cell
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