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商品详细Dojindo/-Bacstain- PI Solution/100/BS07
Dojindo/-Bacstain- PI Solution/100/BS07
Dojindo/-Bacstain- PI Solution/100/BS07
商品编号: BS07
品牌: 同仁
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionApplicationReferencesDataManualS.D.S

Chemical Structure

Staining procedure1. Allow PI solution a) to stand at room temperature for 30 minutes to thaw. Solution should be protected from light.2. Resuspend the organisms with PBS(-) or saline and adjust the number of cells to 106 cells/mL(flow cytometry) or 108-109 cells/mL(microscopy).3. Add 10 μl of PI solution into 1 mL of microbial cell suspension and vortex gently to mix. Formaldehyde-fixation may be carried out, if necessary.4. Incubate the microbial cells at room temperature for 5 minutes.5. Analyze the stained-cells with a flow cytometer or a microscope. The maximum wavelengths of the dye are 530 nm for excitation and 620 nm for emission.

a) Since PI may be carcinogenic, be careful when handling and disposing.

1. N. Yamaguchi, et al., Flow cytometric analysis of bacterial respiratory and enzymatic activity in the natural aquatic environment. J Appl Microbiol. 1997;83:43-52.

Staining Data

Fig. 2S. epidermidis stained with CFDA and PI (left).E. coli stained with CFDA and PI (middle).S. epidermidis stained with DAPI and PI (right).

Related Categories Microbial Viability Assay

品牌介绍
Dojindo细胞分析细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐,称为WST。WST-8是高度稳定的WST,用于Cell Counting Kit-8(CCK-8)。由于WST-8甲maz是水溶性的,因此不会形成晶体。因此,不需要诸如MTT测定的增溶过程。此外,CCK-8的检测灵敏度高于其他四唑盐,例如MTT,XTT,MTS或WST-1。WST检测机制 ß-半乳糖苷酶检测试剂细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢应用产品展示细胞生长检测,药物筛选,比色/荧光检测细胞计数试剂盒-8细胞计数试剂盒8 + 96孔有机硅定向剂细胞计数试剂盒-F细胞毒性LDH检测试剂盒-WST 96孔有机硅定向剂MTT了解检测机制的差异:点击这里细胞周期分析细胞周期测定溶液深红色细胞周期测定溶液蓝色